Journal: Journal of Animal Science and Biotechnology

Article Title: Heat stress induced testicular impairment is related to orchitis and complement activation in Rongchang boars

doi: 10.1186/s40104-025-01296-5

Figure Lengend Snippet: Testicular immunity and orchitis under HS conditions. A and B GO (A) and KEGG (B) enrichments based on Gene Set Enrichment analysis. C Protein-protein interaction network. D Representative photos of collagen fibers stained by Masson in the testes. E and F Levels of TNF-α (E) and IL-1β (F) in plasma isolated from the spermatic vein. G Representative flow cytometry quantifications of macrophages in the testes. H and I Percentages of CD68 + CD163 + and CD68 + cells among CD45 + cells in the testes. * P < 0.05, ** P < 0.01

Article Snippet: The remaining cells were resuspended in 100 μL Stain Buffer (BD, Franklin Lakes, New Jersey, USA) containing mouse anti-pig CD45: FITC (Bio-Rad, Hercules, California, USA), mouse anti-pig CD163: RPE (Bio-Rad, Hercules, California, USA), and BV421 rat anti-CD11b (BD, Franklin Lakes, New Jersey, USA) at 4 °C for 30 min. Each suspension was centrifuged at 350 × g for 5 min at 4 °C to remove the supernatant.

Techniques: Staining, Clinical Proteomics, Isolation, Flow Cytometry

Journal: Journal of Animal Science and Biotechnology

Article Title: Heat stress induced testicular impairment is related to orchitis and complement activation in Rongchang boars

doi: 10.1186/s40104-025-01296-5

Figure Lengend Snippet: Changes in the TM profile and complement pathway under HS conditions. A tSNE graph of identified cells. B Percentages of cells in each group. C KEGG enrichment of the DEGs from TMs. D Expression levels of C1QA , C1QB , and C1QC in testicular cells as assessed by scRNA-seq. E tSNE graph of TM subclusters in each group. F Percentages of subclusters in each group. G KEGG enrichment of marker genes in Subcluster 5. H Expression levels of C1QA , C1QB , and C1QC in TM subclusters as assessed by scRNA-seq. I KEGG enrichment of DEGs from TM Subcluster 5. J Addmodulescore of subclusters. K tSNE graph of C1QA , C1QB , C1QC , and CD163 as assessed by scRNA-seq

Article Snippet: The remaining cells were resuspended in 100 μL Stain Buffer (BD, Franklin Lakes, New Jersey, USA) containing mouse anti-pig CD45: FITC (Bio-Rad, Hercules, California, USA), mouse anti-pig CD163: RPE (Bio-Rad, Hercules, California, USA), and BV421 rat anti-CD11b (BD, Franklin Lakes, New Jersey, USA) at 4 °C for 30 min. Each suspension was centrifuged at 350 × g for 5 min at 4 °C to remove the supernatant.

Techniques: Expressing, Marker

Journal: The FASEB Journal

Article Title: Proangiogenic effects of tumor cells on endothelial progenitor cells vary with tumor type in an in vitro and in vivo rat model

doi: 10.1096/fj.201800135rr

Figure Lengend Snippet: Figure 8. Immunohistochemical staining for tumor associated macrophages and tumor cells in tumor xenografts encapsulated in alginate/ fibrin hydrogels. A) Representative images of CD68, CD163, and cytokeratin immunohisto- chemical staining in CC531 and MCR86 groups. B) Representative images of CD68, CD163, and vimentin immunohistochemical staining in ROS-1 group. Scale bars, 100 mm.

Article Snippet: To whether investigate macrophages infiltrated the implanted hydrogels, an anti-rat CD68 mouse monoclonal antibody (1:300, clone ED1) and CD163 (1:200, clone ED2; both from Bio-Rad) were used after pretreatment with pronase (MilliporeSigma).

Techniques: Immunohistochemical staining, Staining

Journal: Veterinary Research

Article Title: Distribution of porcine monocytes in different lymphoid tissues and the lungs during experimental Actinobacillus pleuropneumoniae infection and the role of chemokines

doi: 10.1186/1297-9716-44-98

Figure Lengend Snippet: Overview of flow cytometry analyses. (A) Gating strategy for identification of monocytes and macrophages. The peripheral blood is shown. Designation of population shown within each dot-plot is indicated above the dot-plot. Leukocytes were identified as viable (a) non-doublet (b) cells with typical light scatter properties of leukocytes (c) . Then, macrophages were gated simply as CD203a hi leukocytes (d) and marked with blue color. Monocytes were gated as CD203a low/- SWC8 - (e) CD172a hi (f) leukocytes where the CD203a low/- region was defined as the complementary region to the CD203a hi region. Then, SLA-DR + monocytes were marked with red color and SLA-DR - monocytes were marked with green color (g) . SLA-DR - region was defined as the complementary region to the SLA-DR + region. Gating order is shown in the scheme (h) . (B) Representative CD163 vs. CD14 dot-plots of macrophages (blue) and monocyte subpopulations (green: SLA-DR – , red: SLA-DR + ) in various body compartments of control and APP-infected pigs.

Article Snippet: BM and PB mononuclear cells, freshly isolated from BM and PB samples by Lympholyte-H gradient (Cedarlane, Burlington, Canada) centrifugation at 800 g , were labeled with anti-CD163 antibody for 15 min at 4 °C.

Techniques: Flow Cytometry, Infection

Journal: Veterinary Research

Article Title: Distribution of porcine monocytes in different lymphoid tissues and the lungs during experimental Actinobacillus pleuropneumoniae infection and the role of chemokines

doi: 10.1186/1297-9716-44-98

Figure Lengend Snippet: Primers for chemokines, chemokine receptors, CD62L and reference gene used in quantitative real-time PCR.

Article Snippet: BM and PB mononuclear cells, freshly isolated from BM and PB samples by Lympholyte-H gradient (Cedarlane, Burlington, Canada) centrifugation at 800 g , were labeled with anti-CD163 antibody for 15 min at 4 °C.

Techniques:

Journal: Veterinary Research

Article Title: Distribution of porcine monocytes in different lymphoid tissues and the lungs during experimental Actinobacillus pleuropneumoniae infection and the role of chemokines

doi: 10.1186/1297-9716-44-98

Figure Lengend Snippet: Monocyte subpopulations in various body compartments from control and APP-infected pigs.

Article Snippet: BM and PB mononuclear cells, freshly isolated from BM and PB samples by Lympholyte-H gradient (Cedarlane, Burlington, Canada) centrifugation at 800 g , were labeled with anti-CD163 antibody for 15 min at 4 °C.

Techniques:

Journal: Veterinary Research

Article Title: Distribution of porcine monocytes in different lymphoid tissues and the lungs during experimental Actinobacillus pleuropneumoniae infection and the role of chemokines

doi: 10.1186/1297-9716-44-98

Figure Lengend Snippet: Representative pictures of immunohistochemical detection of CD163 + cells in tracheobronchial lymph node and spleen. CD163 + cells were detected in tracheobronchial lymph nodes from control (A) and APP-infected pigs (B) and in spleen from control (C) and APP-infected pigs (D) . Immunohistochemical visualization: horseradish peroxidase, brown substrate, hematoxylin counterstain; c, cortex; ca, central artery; e, ellipsoid; f, follicle; mz, marginal zone; pals, periarterial lymphatic sheath, rp, red pulp; s, subcapsular sinus.

Article Snippet: BM and PB mononuclear cells, freshly isolated from BM and PB samples by Lympholyte-H gradient (Cedarlane, Burlington, Canada) centrifugation at 800 g , were labeled with anti-CD163 antibody for 15 min at 4 °C.

Techniques: Immunohistochemical staining, Infection

Journal: Veterinary Research

Article Title: Distribution of porcine monocytes in different lymphoid tissues and the lungs during experimental Actinobacillus pleuropneumoniae infection and the role of chemokines

doi: 10.1186/1297-9716-44-98

Figure Lengend Snippet: Intensity of CD163, CD14 and SLA-DR expression by CD14 + /CD163 + monocytes in various body compartments. Data are shown as MFI (Median of fluorescence intensity) ± S.E.M. BM, bone marrow ( n = 5); PB, peripheral blood ( n = 5), UA, lungs-unaffected area ( n = 5), DZ, lungs-demarcation zone ( n = 4), NA, lungs-necrotic area ( n = 5), TBLN, tracheobronchial lymph node ( n = 5); MLN, mesenteric lymph node ( n = 4), spleen ( n = 4). The significant differences (Kruskal-Wallis test) amongst particular compartments are indicated.

Article Snippet: BM and PB mononuclear cells, freshly isolated from BM and PB samples by Lympholyte-H gradient (Cedarlane, Burlington, Canada) centrifugation at 800 g , were labeled with anti-CD163 antibody for 15 min at 4 °C.

Techniques: Expressing, Fluorescence

Journal: Veterinary Research

Article Title: Distribution of porcine monocytes in different lymphoid tissues and the lungs during experimental Actinobacillus pleuropneumoniae infection and the role of chemokines

doi: 10.1186/1297-9716-44-98

Figure Lengend Snippet: Chemokine receptor, CD62L, CD163 and TBP1 expression by bone marrow and peripheral blood CD163 + monocytes from control and APP-infected pigs.

Article Snippet: BM and PB mononuclear cells, freshly isolated from BM and PB samples by Lympholyte-H gradient (Cedarlane, Burlington, Canada) centrifugation at 800 g , were labeled with anti-CD163 antibody for 15 min at 4 °C.

Techniques: Expressing

Journal: Veterinary Research

Article Title: Distribution of porcine monocytes in different lymphoid tissues and the lungs during experimental Actinobacillus pleuropneumoniae infection and the role of chemokines

doi: 10.1186/1297-9716-44-98

Figure Lengend Snippet: Chemokine receptor, CD62L and CD163 expression in lungs from control and APP-infected pigs.

Article Snippet: BM and PB mononuclear cells, freshly isolated from BM and PB samples by Lympholyte-H gradient (Cedarlane, Burlington, Canada) centrifugation at 800 g , were labeled with anti-CD163 antibody for 15 min at 4 °C.

Techniques: Expressing

Journal: Veterinary Research

Article Title: Distribution of porcine monocytes in different lymphoid tissues and the lungs during experimental Actinobacillus pleuropneumoniae infection and the role of chemokines

doi: 10.1186/1297-9716-44-98

Figure Lengend Snippet: Scheme of chemokines and corresponding chemokine receptor mRNA expression in various organs and CD163 + monocytes. Chemokines in bone marrow, necrotic area of the lungs and tracheobronchial lymph nodes and chemokine receptors in necrotic areas of the lungs and tracheobronchial lymph nodes which were up-regulated in the APP-infected pigs compared to control are highlighted in bold. Chemokine receptors in bone marrow and peripheral blood CD163 + monocytes which were expressed at relatively lower levels are highlighted in bold. Those expressed in relatively high levels are highlighted in bold and underlined. Arrangement of the scheme was created based on the review of Bonecchi et al. .

Article Snippet: BM and PB mononuclear cells, freshly isolated from BM and PB samples by Lympholyte-H gradient (Cedarlane, Burlington, Canada) centrifugation at 800 g , were labeled with anti-CD163 antibody for 15 min at 4 °C.

Techniques: Expressing, Infection

Journal: World Journal of Surgical Oncology

Article Title: A study of the correlation between M2 macrophages and lymph node metastasis of colorectal carcinoma

doi: 10.1186/s12957-021-02195-5

Figure Lengend Snippet: Expression of M2 macrophages in lymph nodes. Detection of CD163 in lymph nodes of colorectal carcinoma patients by immunohistochemistry. The membrane and cytoplasm of M2 macrophages are stained brown. Microscopic analysis of a typical example of CD163 expression in non-metastatic lymph nodes ( a , b , c magnification × 100, × 200, × 400, respectively). d , e Location of CD163 in metastatic lymph node tissue at a magnification of × 200 and × 400 respectively. M2 macrophages are seen mainly infiltrating into the tumor stroma

Article Snippet: The tissue sections were incubated with an endogenous peroxidase blocker at room temperature for 10 min, blocked with 3% goat serum (cat. No. KIT-9710; Maixin-Bio, Fuzhou, China) for 30 min, rinsed with phosphate-buffered saline (PBS), incubated with ready-to-use mouse anti-human CD163 monoclonal antibody (cat. No. MAB-0206; Maixin-Bio) at room temperature for 60 min, followed by sequential addition of biotin-labeled IgG polymer and streptavidin peroxidase (cat. No. KIT-9710; Maixin-Bio).

Techniques: Expressing, Immunohistochemistry, Staining